Characterization of Substitution Mutations of eIF4G Gene Generated through Adenine Base Editors in Rice

Adenine base editor (ABE) creates A to G transitions within its editing window. In the present study, an ABE was used target a stretch of six amino acid residues, VLFPNL in translation initiation factor four gamma (eIF4G) gene rice. Agrobacterium-mediated transformation rice cultivar ASD16 resulted T0 events with high mutation efficiency 89.29 %. Substitution mutations > occurred window eight bases at A7 G7 (74.67 %) and A4 G4 (2.46 %). Non-canonical substitutions C/A also observed G15 C15 (9.29 G8 A8 (1.15 total 15 missense substitution affecting residue identified. Taken together, study showed that ABEs create unexpected besides efficient canonical genome